diff --git a/js/common/lib/inference-session.ts b/js/common/lib/inference-session.ts
index edc32535fc..1221b52cd4 100644
--- a/js/common/lib/inference-session.ts
+++ b/js/common/lib/inference-session.ts
@@ -181,7 +181,7 @@ export declare namespace InferenceSession {
 
   // Currently, we have the following backends to support execution providers:
   // Backend Node.js binding: supports 'cpu' and 'cuda'.
-  // Backend WebAssembly: supports 'cpu', 'wasm', 'xnnpack' and 'webnn'.
+  // Backend WebAssembly: supports 'cpu', 'wasm', 'webgpu' and 'webnn'.
   // Backend ONNX.js: supports 'webgl'.
   // Backend React Native: supports 'cpu', 'xnnpack', 'coreml' (iOS), 'nnapi' (Android).
   interface ExecutionProviderOptionMap {
diff --git a/js/web/lib/index.ts b/js/web/lib/index.ts
index 4f1a3943de..baf45e74ad 100644
--- a/js/web/lib/index.ts
+++ b/js/web/lib/index.ts
@@ -26,11 +26,8 @@ if (!BUILD_DEFS.DISABLE_WASM) {
   }
   registerBackend('cpu', wasmBackend, 10);
   registerBackend('wasm', wasmBackend, 10);
-  if (BUILD_DEFS.DISABLE_TRAINING) {
-    registerBackend('xnnpack', wasmBackend, 9);
-    if (!BUILD_DEFS.DISABLE_WEBNN) {
-      registerBackend('webnn', wasmBackend, 9);
-    }
+  if (!BUILD_DEFS.DISABLE_WEBNN) {
+    registerBackend('webnn', wasmBackend, 9);
   }
 }
 
diff --git a/js/web/lib/wasm/session-options.ts b/js/web/lib/wasm/session-options.ts
index 45ea48a2df..41ab2d52ca 100644
--- a/js/web/lib/wasm/session-options.ts
+++ b/js/web/lib/wasm/session-options.ts
@@ -60,9 +60,6 @@ const setExecutionProviders =
 
         // check EP name
         switch (epName) {
-          case 'xnnpack':
-            epName = 'XNNPACK';
-            break;
           case 'webnn':
             epName = 'WEBNN';
             if (typeof ep !== 'string') {
diff --git a/js/web/script/test-runner-cli-args.ts b/js/web/script/test-runner-cli-args.ts
index fc74adfed1..8f6c5f6f04 100644
--- a/js/web/script/test-runner-cli-args.ts
+++ b/js/web/script/test-runner-cli-args.ts
@@ -36,7 +36,6 @@ Options:
                                    webgl
                                    webgpu
                                    wasm
-                                   xnnpack
                                    webnn
  -e=<...>, --env=<...>         Specify the environment to run the test. Should be one of the following:
                                  chrome     (default)
@@ -111,7 +110,7 @@ Examples:
 
 export declare namespace TestRunnerCliArgs {
   type Mode = 'suite0'|'suite1'|'model'|'unittest'|'op';
-  type Backend = 'cpu'|'webgl'|'webgpu'|'wasm'|'onnxruntime'|'xnnpack'|'webnn';
+  type Backend = 'cpu'|'webgl'|'webgpu'|'wasm'|'onnxruntime'|'webnn';
   type Environment = 'chrome'|'edge'|'firefox'|'electron'|'safari'|'node'|'bs';
   type BundleMode = 'dev'|'perf';
   type IOBindingMode = 'none'|'gpu-tensor'|'gpu-location';
@@ -378,13 +377,13 @@ export function parseTestRunnerCliArgs(cmdlineArgs: string[]): TestRunnerCliArgs
   }
 
   // Option: -b=<...>, --backend=<...>
-  const browserBackends = ['webgl', 'webgpu', 'wasm', 'xnnpack', 'webnn'];
+  const browserBackends = ['webgl', 'webgpu', 'wasm', 'webnn'];
 
   // TODO: remove this when Chrome support WebNN.
   //       we need this for now because Chrome does not support webnn yet,
   //       and ChromeCanary is not in CI.
 
-  const defaultBrowserBackends = ['webgl', 'webgpu', 'wasm', 'xnnpack' /*, 'webnn'*/];
+  const defaultBrowserBackends = ['webgl', 'webgpu', 'wasm' /*, 'webnn'*/];
   const nodejsBackends = ['cpu', 'wasm'];
   const backendArgs = args.backend || args.b;
   const backend = (typeof backendArgs !== 'string') ? (env === 'node' ? nodejsBackends : defaultBrowserBackends) :
diff --git a/js/web/test/test-runner.ts b/js/web/test/test-runner.ts
index 3492c8f378..442cb1bcf1 100644
--- a/js/web/test/test-runner.ts
+++ b/js/web/test/test-runner.ts
@@ -96,7 +96,7 @@ async function loadTensors(
   const outputs: Test.NamedTensor[] = [];
   let dataFileType: 'none'|'pb'|'npy' = 'none';
 
-  const allowInt64 = ['wasm', 'xnnpack', 'webgpu', 'webnn'].includes(backendName);
+  const allowInt64 = ['wasm', 'webgpu', 'webnn'].includes(backendName);
 
   for (const dataFile of testCase.dataFiles) {
     const ext = extname(dataFile);
@@ -317,7 +317,7 @@ export class TensorResultValidator {
     } else if (backend === 'webgpu') {
       this.absoluteThreshold = WEBGPU_THRESHOLD_ABSOLUTE_ERROR;
       this.relativeThreshold = WEBGPU_THRESHOLD_RELATIVE_ERROR;
-    } else if (backend === 'wasm' || backend === 'xnnpack' || backend === 'webnn') {
+    } else if (backend === 'wasm' || backend === 'webnn') {
       this.absoluteThreshold = WASM_THRESHOLD_ABSOLUTE_ERROR;
       this.relativeThreshold = WASM_THRESHOLD_RELATIVE_ERROR;
     } else if (backend === 'onnxruntime') {
diff --git a/tools/ci_build/github/azure-pipelines/templates/win-web-ci.yml b/tools/ci_build/github/azure-pipelines/templates/win-web-ci.yml
index 8d4efc79ea..8ba3517530 100644
--- a/tools/ci_build/github/azure-pipelines/templates/win-web-ci.yml
+++ b/tools/ci_build/github/azure-pipelines/templates/win-web-ci.yml
@@ -169,12 +169,12 @@ jobs:
       errorActionPreference: stop
     displayName: 'Pack NPM packages'
   - script: |
-     npm test -- -e=chrome -b=webgl,wasm,xnnpack
+     npm test -- -e=chrome -b=webgl,wasm
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
-    displayName: 'Run ort-web tests (wasm,webgl,xnnpack backend)'
+    displayName: 'Run ort-web tests (wasm,webgl backend)'
     condition: eq('${{ parameters.RunWebGpuTests }}', 'false')
   - script: |
-     npm test -- -e=chrome -b=webgl,wasm,xnnpack,webgpu $(webgpuCommandlineExtraFlags)
+     npm test -- -e=chrome -b=webgl,wasm,webgpu $(webgpuCommandlineExtraFlags)
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
     displayName: 'Run ort-web tests (ALL backends)'
     condition: eq('${{ parameters.RunWebGpuTests }}', 'true')
diff --git a/tools/ci_build/github/azure-pipelines/templates/win-web-multi-browsers.yml b/tools/ci_build/github/azure-pipelines/templates/win-web-multi-browsers.yml
index f7876f1502..31ee488318 100644
--- a/tools/ci_build/github/azure-pipelines/templates/win-web-multi-browsers.yml
+++ b/tools/ci_build/github/azure-pipelines/templates/win-web-multi-browsers.yml
@@ -68,15 +68,15 @@ jobs:
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
     displayName: 'npm ci /js/web/'
   - script: |
-      npm test -- suite0 -b=wasm,webgl,xnnpack --wasm-init-timeout=30000 --file-cache
+      npm test -- suite0 -b=wasm,webgl --wasm-init-timeout=30000 --file-cache
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
     displayName: 'npm test (Suite0, Chrome)'
   - script: |
-      npm test -- suite0 -b=wasm,webgl,xnnpack --env=firefox --wasm-init-timeout=30000 --file-cache
+      npm test -- suite0 -b=wasm,webgl --env=firefox --wasm-init-timeout=30000 --file-cache
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
     displayName: 'npm test (Suite0, Firefox)'
   - script: |
-      npm test -- suite0 -b=wasm,webgl,xnnpack --env=edge --wasm-init-timeout=30000 --file-cache
+      npm test -- suite0 -b=wasm,webgl --env=edge --wasm-init-timeout=30000 --file-cache
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
     displayName: 'npm test (Suite0, Edge)'
   - task: mspremier.PostBuildCleanup.PostBuildCleanup-task.PostBuildCleanup@3