diff --git a/js/web/lib/wasm/proxy-wrapper.ts b/js/web/lib/wasm/proxy-wrapper.ts
index 12b5b9287a..105f46c43f 100644
--- a/js/web/lib/wasm/proxy-wrapper.ts
+++ b/js/web/lib/wasm/proxy-wrapper.ts
@@ -79,7 +79,7 @@ const onProxyWorkerMessage = (ev: MessageEvent<OrtWasmMessage>): void => {
   }
 };
 
-const scriptSrc = isProxy() ? (document?.currentScript as HTMLScriptElement)?.src : undefined;
+const scriptSrc = typeof document !== 'undefined' ? (document?.currentScript as HTMLScriptElement)?.src : undefined;
 
 export const initWasm = async(): Promise<void> => {
   if (isProxy()) {
diff --git a/js/web/test/e2e/browser-test-wasm-no-threads-proxy.js b/js/web/test/e2e/browser-test-wasm-no-threads-proxy.js
new file mode 100644
index 0000000000..d9e4a66d1d
--- /dev/null
+++ b/js/web/test/e2e/browser-test-wasm-no-threads-proxy.js
@@ -0,0 +1,10 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+'use strict';
+
+it('Browser E2E testing - WebAssembly backend (no threads, proxy)', async function () {
+  ort.env.wasm.numThreads = 1;
+  ort.env.wasm.proxy = true;
+  await testFunction(ort, { executionProviders: ['wasm'] });
+});
diff --git a/js/web/test/e2e/browser-test-wasm-proxy.js b/js/web/test/e2e/browser-test-wasm-proxy.js
new file mode 100644
index 0000000000..eae66a23ec
--- /dev/null
+++ b/js/web/test/e2e/browser-test-wasm-proxy.js
@@ -0,0 +1,9 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+'use strict';
+
+it('Browser E2E testing - WebAssembly backend (proxy)', async function () {
+  ort.env.wasm.proxy = true;
+  await testFunction(ort, { executionProviders: ['wasm'] });
+});
diff --git a/js/web/test/e2e/karma.conf.js b/js/web/test/e2e/karma.conf.js
index 8468fb722b..42ca6e0313 100644
--- a/js/web/test/e2e/karma.conf.js
+++ b/js/web/test/e2e/karma.conf.js
@@ -19,7 +19,7 @@ module.exports = function (config) {
   config.set({
     frameworks: ['mocha'],
     files: [
-      { pattern: distPrefix + 'ort.js' },
+      { pattern: distPrefix + 'ort.min.js' },
       { pattern: './common.js' },
       { pattern: TEST_MAIN },
       { pattern: './node_modules/onnxruntime-web/dist/*.wasm', included: false, nocache: true },
diff --git a/js/web/test/e2e/run.js b/js/web/test/e2e/run.js
index 095004d7a3..468d4611c5 100644
--- a/js/web/test/e2e/run.js
+++ b/js/web/test/e2e/run.js
@@ -86,6 +86,8 @@ async function testAllBrowserCases({ hostInKarma }) {
   await runKarma({ hostInKarma, main: './browser-test-webgl.js', browser: 'Chrome_default' });
   await runKarma({ hostInKarma, main: './browser-test-wasm.js', browser: 'Chrome_default' });
   await runKarma({ hostInKarma, main: './browser-test-wasm-no-threads.js', browser: 'Chrome_default' });
+  await runKarma({ hostInKarma, main: './browser-test-wasm-proxy.js', browser: 'Chrome_default' });
+  await runKarma({ hostInKarma, main: './browser-test-wasm-no-threads-proxy.js', browser: 'Chrome_default' });
   await runKarma({ hostInKarma, main: './browser-test-wasm-path-override-filename.js', browser: 'Chrome_default' });
   await runKarma({ hostInKarma, main: './browser-test-wasm-path-override-prefix.js', browser: 'Chrome_default' });
 }
diff --git a/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml b/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml
index aa6ba8afeb..e3c7a09956 100644
--- a/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml
+++ b/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml
@@ -217,11 +217,15 @@ jobs:
   - script: |
      npm test
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
-    displayName: 'Run ort-web tests - unpacked mode'
+    displayName: 'Run ort-web tests'
   - script: |
      npm test -- --webgl-texture-pack-mode -b=webgl
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
-    displayName: 'Run ort-web tests - packed mode'
+    displayName: 'Run ort-web tests - WebGL: packed mode'
+  - script: |
+     npm test -- --wasm-enable-proxy -b=wasm
+    workingDirectory: '$(Build.SourcesDirectory)\js\web'
+    displayName: 'Run ort-web tests - WebAssembly: proxy'
   - script: |
       npm pack
     workingDirectory: '$(Build.SourcesDirectory)\js\common'