diff --git a/js/web/lib/wasm/jsep/webgpu/ops/conv.ts b/js/web/lib/wasm/jsep/webgpu/ops/conv.ts
index cb40a9f08d..7af2c5db49 100644
--- a/js/web/lib/wasm/jsep/webgpu/ops/conv.ts
+++ b/js/web/lib/wasm/jsep/webgpu/ops/conv.ts
@@ -138,8 +138,12 @@ const conv2d = (context: ComputeContext, inputs: readonly TensorView[], attribut
   // const hasPreluActivationWeights = false; /* TODO: add support for prelu activation weights */
   const isChannelsLast = attributes.format === 'NHWC';
   if (attributes.group !== 1) {
-    if (isChannelsLast && inputs[1].dims[0] === attributes.group && inputs[1].dims[1] === 1 &&
-        attributes.dilations[0] === 1 && attributes.dilations[1] === 1) {
+    // Temporarily disable createGroupedConvVectorizeProgramInfo path due to bots failures with below two cases:
+    // [webgpu]Conv - conv - vectorize group - B
+    // [webgpu]Conv - conv - vectorize group - D
+    const disableGroupedConvVectorize = true;
+    if (!disableGroupedConvVectorize && isChannelsLast && inputs[1].dims[0] === attributes.group &&
+        inputs[1].dims[1] === 1 && attributes.dilations[0] === 1 && attributes.dilations[1] === 1) {
       const outputShape = calculateOutputShape(
           inputs[0].dims, inputs[1].dims, attributes.dilations, adjustedAttributes.pads, attributes.strides,
           isChannelsLast);