diff --git a/js/README.md b/js/README.md
index 5ef449b3a0..488b025cc6 100644
--- a/js/README.md
+++ b/js/README.md
@@ -127,6 +127,7 @@ Node.js v12+ (recommended v14+)
 2. ~~Follow [instructions](https://www.onnxruntime.ai/docs/how-to/build.html#apis-and-language-bindings) for building ONNX Runtime WebAssembly. (TODO: document is not ready. we are working on it.)~~
 
    in `<ORT_ROOT>/`, run either of the following commands to build WebAssembly:
+
    ```sh
    # In windows, use 'build' to replace './build.sh'
 
@@ -136,16 +137,19 @@ Node.js v12+ (recommended v14+)
    # The following command build release.
    ./build.sh --config Release --build_wasm --skip_tests --disable_wasm_exception_catching --disable_rtti
    ```
+
    To build with multi-thread support, append flag ` --enable_wasm_threads` to the command.
 
 3. Copy following files from build output folder to `<ORT_ROOT>/js/web/dist/`:
+
    - ort-wasm.wasm
-   - ort-wasm-threaded.wasm (if appliable)
-   - ort-wasm-threaded.worker.js (if appliable)
+   - ort-wasm-threaded.wasm (build with flag '--enable_wasm_threads')
 
 4. Copy following files from build output folder to `<ORT_ROOT>/js/web/lib/wasm/binding/`:
+
    - ort-wasm.js
-   - ort-wasm-threaded.js (if appliable)
+   - ort-wasm-threaded.js (build with flag '--enable_wasm_threads')
+   - ort-wasm-threaded.worker.js (build with flag '--enable_wasm_threads')
 
 5. Use following command in folder `<ORT_ROOT>/js/web` to build:
    ```
diff --git a/js/web/.gitignore b/js/web/.gitignore
index 4ec6ca918a..e6da0a8e2d 100644
--- a/js/web/.gitignore
+++ b/js/web/.gitignore
@@ -16,4 +16,5 @@ script/**/*.js.map
 lib/wasm/binding/**/*.wasm
 !lib/wasm/binding/**/*.d.ts
 
+test/testdata-config.json
 test/data/node/
diff --git a/js/web/karma.conf.js b/js/web/karma.conf.js
index 5b8991093b..d7900a3124 100644
--- a/js/web/karma.conf.js
+++ b/js/web/karma.conf.js
@@ -45,10 +45,8 @@ module.exports = function (config) {
     frameworks: ['mocha'],
     files: [
       { pattern: commonFile },
-      { pattern: 'test/testdata-config.js' },
       { pattern: mainFile },
       { pattern: 'test/testdata-file-cache-*.json', included: false },
-      //{ pattern: 'test/onnx-worker.js', included: false },
       { pattern: 'test/data/**/*', included: false, nocache: true },
       { pattern: 'dist/ort-wasm.wasm', included: false },
       { pattern: 'dist/ort-wasm-threaded.wasm', included: false },
diff --git a/js/web/lib/backend-wasm.ts b/js/web/lib/backend-wasm.ts
index 9192a2ebd6..ee5d10fca8 100644
--- a/js/web/lib/backend-wasm.ts
+++ b/js/web/lib/backend-wasm.ts
@@ -1,7 +1,10 @@
 // Copyright (c) Microsoft Corporation. All rights reserved.
 // Licensed under the MIT License.
 
+import {readFile} from 'fs';
 import {Backend, env, InferenceSession, SessionHandler} from 'onnxruntime-common';
+import {cpus} from 'os';
+import {promisify} from 'util';
 
 import {OnnxruntimeWebAssemblySessionHandler} from './wasm/session-handler';
 import {initializeWebAssembly} from './wasm/wasm-factory';
@@ -18,7 +21,8 @@ export const initializeFlags = (): void => {
   }
 
   if (typeof env.wasm.numThreads !== 'number' || !Number.isInteger(env.wasm.numThreads) || env.wasm.numThreads < 0) {
-    env.wasm.numThreads = Math.ceil((navigator.hardwareConcurrency || 1) / 2);
+    const numCpuLogicalCores = typeof navigator === 'undefined' ? cpus().length : navigator.hardwareConcurrency;
+    env.wasm.numThreads = Math.ceil((numCpuLogicalCores || 1) / 2);
   }
   env.wasm.numThreads = Math.min(4, env.wasm.numThreads);
 
@@ -42,9 +46,15 @@ class OnnxruntimeWebAssemblyBackend implements Backend {
       Promise<SessionHandler> {
     let buffer: Uint8Array;
     if (typeof pathOrBuffer === 'string') {
-      const response = await fetch(pathOrBuffer);
-      const arrayBuffer = await response.arrayBuffer();
-      buffer = new Uint8Array(arrayBuffer);
+      if (typeof fetch === 'undefined') {
+        // node
+        buffer = await promisify(readFile)(pathOrBuffer);
+      } else {
+        // browser
+        const response = await fetch(pathOrBuffer);
+        const arrayBuffer = await response.arrayBuffer();
+        buffer = new Uint8Array(arrayBuffer);
+      }
     } else {
       buffer = pathOrBuffer;
     }
diff --git a/js/web/lib/wasm/binding/ort-wasm-threaded.d.ts b/js/web/lib/wasm/binding/ort-wasm-threaded.d.ts
index ad8e5f6636..b5898908b2 100644
--- a/js/web/lib/wasm/binding/ort-wasm-threaded.d.ts
+++ b/js/web/lib/wasm/binding/ort-wasm-threaded.d.ts
@@ -3,5 +3,9 @@
 
 import {OrtWasmModule} from './ort-wasm';
 
-declare const moduleFactory: EmscriptenModuleFactory<OrtWasmModule>;
+export interface OrtWasmThreadedModule extends OrtWasmModule {
+  PThread?: {terminateAllThreads(): void};
+}
+
+declare const moduleFactory: EmscriptenModuleFactory<OrtWasmThreadedModule>;
 export default moduleFactory;
diff --git a/js/web/lib/wasm/wasm-factory.ts b/js/web/lib/wasm/wasm-factory.ts
index c7bef35eaf..b51a85d003 100644
--- a/js/web/lib/wasm/wasm-factory.ts
+++ b/js/web/lib/wasm/wasm-factory.ts
@@ -2,7 +2,10 @@
 // Licensed under the MIT License.
 
 import {env} from 'onnxruntime-common';
+import * as path from 'path';
+
 import {OrtWasmModule} from './binding/ort-wasm';
+import {OrtWasmThreadedModule} from './binding/ort-wasm-threaded';
 import ortWasmFactoryThreaded from './binding/ort-wasm-threaded.js';
 import ortWasmFactory from './binding/ort-wasm.js';
 
@@ -13,11 +16,14 @@ let aborted = false;
 
 const isMultiThreadSupported = (): boolean => {
   try {
-    // Test for transferability of SABs (needed for Firefox)
+    // Test for transferability of SABs (for browsers. needed for Firefox)
     // https://groups.google.com/forum/#!msg/mozilla.dev.platform/IHkBZlHETpA/dwsMNchWEQAJ
-    new MessageChannel().port1.postMessage(new SharedArrayBuffer(1));
-    // This typed array is a WebAssembly program containing threaded
-    // instructions.
+    if (typeof MessageChannel !== 'undefined') {
+      new MessageChannel().port1.postMessage(new SharedArrayBuffer(1));
+    }
+
+    // Test for WebAssembly threads capability (for both browsers and Node.js)
+    // This typed array is a WebAssembly program containing threaded instructions.
     return WebAssembly.validate(new Uint8Array([
       0, 97, 115, 109, 1, 0,  0,  0, 1, 4, 1,  96, 0,   0,  3, 2, 1,  0, 5,
       4, 1,  3,   1,   1, 10, 11, 1, 9, 0, 65, 0,  254, 16, 2, 0, 26, 11
@@ -65,9 +71,25 @@ export const initializeWebAssembly = async(): Promise<void> => {
     const config: Partial<OrtWasmModule> = {};
 
     if (useThreads) {
-      config.mainScriptUrlOrBlob = new Blob(
-          [`var ortWasmThreaded=(function(){var _scriptDir;return ${ortWasmFactoryThreaded.toString()}})();`],
-          {type: 'text/javascript'});
+      if (typeof Blob === 'undefined') {
+        config.mainScriptUrlOrBlob = path.join(__dirname, 'ort-wasm-threaded.js');
+      } else {
+        const scriptSourceCode =
+            `var ortWasmThreaded=(function(){var _scriptDir;return ${ortWasmFactoryThreaded.toString()}})();`;
+        config.mainScriptUrlOrBlob = new Blob([scriptSourceCode], {type: 'text/javascript'});
+        config.locateFile = (fileName: string, scriptDirectory: string) => {
+          if (fileName.endsWith('.worker.js')) {
+            return URL.createObjectURL(new Blob(
+                [
+                  // This require() function is handled by webpack to load file content of the corresponding .worker.js
+                  // eslint-disable-next-line @typescript-eslint/no-require-imports
+                  require('./binding/ort-wasm-threaded.worker.js')
+                ],
+                {type: 'text/javascript'}));
+          }
+          return scriptDirectory + fileName;
+        };
+      }
     }
 
     factory(config).then(
@@ -100,3 +122,15 @@ export const getInstance = (): OrtWasmModule => {
 
   throw new Error('WebAssembly is not initialized yet.');
 };
+
+export const dispose = (): void => {
+  if (initialized && !initializing && !aborted) {
+    initializing = true;
+
+    (wasm as OrtWasmThreadedModule).PThread?.terminateAllThreads();
+
+    initializing = false;
+    initialized = false;
+    aborted = true;
+  }
+};
diff --git a/js/web/package.json b/js/web/package.json
index 6cd261c924..053d627305 100644
--- a/js/web/package.json
+++ b/js/web/package.json
@@ -17,6 +17,7 @@
     "prepare": "tsc",
     "build": "node ./script/build",
     "test": "node ./script/prepare-test-data && node ./script/test-runner-cli",
+    "test:e2e": "node ./test/e2e/run",
     "prepack": "node ./script/prepack"
   },
   "dependencies": {
diff --git a/js/web/script/build.ts b/js/web/script/build.ts
index a85ce1a681..9c64b0876d 100644
--- a/js/web/script/build.ts
+++ b/js/web/script/build.ts
@@ -13,8 +13,9 @@ const args = minimist(process.argv);
 // --bundle-mode=prod (default)
 // --bundle-mode=dev
 // --bundle-mode=perf
+// --bundle-mode=node
 const MODE = args['bundle-mode'] || 'prod';
-if (['prod', 'dev', 'perf'].indexOf(MODE) === -1) {
+if (['prod', 'dev', 'perf', 'node'].indexOf(MODE) === -1) {
   throw new Error(`unknown build mode: ${MODE}`);
 }
 
@@ -24,12 +25,16 @@ const WASM = typeof args.wasm === 'undefined' ? true : !!args.wasm;
 
 // Path variables
 const WASM_BINDING_FOLDER = path.join(__dirname, '..', 'lib', 'wasm', 'binding');
-const WASM_JS_PATH = path.join(WASM_BINDING_FOLDER, 'ort-wasm.js');
-const WASM_THREADED_JS_PATH = path.join(WASM_BINDING_FOLDER, 'ort-wasm-threaded.js');
+const WASM_BINDING_JS_PATH = path.join(WASM_BINDING_FOLDER, 'ort-wasm.js');
+const WASM_BINDING_THREADED_JS_PATH = path.join(WASM_BINDING_FOLDER, 'ort-wasm-threaded.js');
+const WASM_BINDING_THREADED_WORKER_JS_PATH = path.join(WASM_BINDING_FOLDER, 'ort-wasm-threaded.worker.js');
+const WASM_BINDING_THREADED_MIN_JS_PATH = path.join(WASM_BINDING_FOLDER, 'ort-wasm-threaded.min.js');
+const WASM_BINDING_THREADED_MIN_WORKER_JS_PATH = path.join(WASM_BINDING_FOLDER, 'ort-wasm-threaded.min.worker.js');
 const WASM_DIST_FOLDER = path.join(__dirname, '..', 'dist');
 const WASM_WASM_PATH = path.join(WASM_DIST_FOLDER, 'ort-wasm.wasm');
 const WASM_THREADED_WASM_PATH = path.join(WASM_DIST_FOLDER, 'ort-wasm-threaded.wasm');
 const WASM_THREADED_WORKER_JS_PATH = path.join(WASM_DIST_FOLDER, 'ort-wasm-threaded.worker.js');
+const WASM_THREADED_JS_PATH = path.join(WASM_DIST_FOLDER, 'ort-wasm-threaded.js');
 
 function validateFile(path: string): void {
   npmlog.info('Build', `Ensure file: ${path}`);
@@ -41,28 +46,87 @@ function validateFile(path: string): void {
   }
 }
 
+npmlog.info('Build.Bundle', 'Retrieving npm bin folder...');
+const npmBin = execSync('npm bin', {encoding: 'utf8'}).trimRight();
+npmlog.info('Build.Bundle', `Retrieving npm bin folder... DONE, folder: ${npmBin}`);
+
 if (WASM) {
   npmlog.info('Build', 'Validating WebAssembly artifacts...');
   try {
-    validateFile(WASM_JS_PATH);
-    validateFile(WASM_THREADED_JS_PATH);
+    validateFile(WASM_BINDING_JS_PATH);
+    validateFile(WASM_BINDING_THREADED_JS_PATH);
+    validateFile(WASM_BINDING_THREADED_WORKER_JS_PATH);
     validateFile(WASM_WASM_PATH);
     validateFile(WASM_THREADED_WASM_PATH);
-    validateFile(WASM_THREADED_WORKER_JS_PATH);
   } catch (e) {
     npmlog.error('Build', `WebAssembly files are not ready. build WASM first. ERR: ${e}`);
     throw e;
   }
   npmlog.info('Build', 'Validating WebAssembly artifacts... DONE');
+
+  const VERSION = require(path.join(__dirname, '../package.json')).version;
+  const COPYRIGHT_BANNER = `/*!
+ * ONNX Runtime Web v${VERSION}
+ * Copyright (c) Microsoft Corporation. All rights reserved.
+ * Licensed under the MIT License.
+ */
+`;
+
+  const terserCommand = path.join(npmBin, 'terser');
+  npmlog.info('Build', 'Minimizing file "ort-wasm-threaded.js"...');
+  try {
+    const terser = spawnSync(
+        terserCommand,
+        [
+          WASM_BINDING_THREADED_JS_PATH, '--compress', 'passes=2', '--format', 'comments=false', '--mangle',
+          'reserved=[_scriptDir]', '--module'
+        ],
+        {shell: true, encoding: 'utf-8'});
+    if (terser.status !== 0) {
+      console.error(terser.error);
+      process.exit(terser.status === null ? undefined : terser.status);
+    }
+
+    fs.writeFileSync(WASM_BINDING_THREADED_MIN_JS_PATH, terser.stdout);
+    fs.writeFileSync(WASM_THREADED_JS_PATH, COPYRIGHT_BANNER + terser.stdout);
+
+    validateFile(WASM_BINDING_THREADED_MIN_JS_PATH);
+    validateFile(WASM_THREADED_JS_PATH);
+  } catch (e) {
+    npmlog.error('Build', `Failed to run terser on ort-wasm-threaded.js. ERR: ${e}`);
+    throw e;
+  }
+  npmlog.info('Build', 'Minimizing file "ort-wasm-threaded.js"... DONE');
+
+  npmlog.info('Build', 'Minimizing file "ort-wasm-threaded.worker.js"...');
+  try {
+    const terser = spawnSync(
+        terserCommand,
+        [
+          WASM_BINDING_THREADED_WORKER_JS_PATH, '--compress', 'passes=2', '--format', 'comments=false', '--mangle',
+          'reserved=[_scriptDir]', '--toplevel'
+        ],
+        {shell: true, encoding: 'utf-8'});
+    if (terser.status !== 0) {
+      console.error(terser.error);
+      process.exit(terser.status === null ? undefined : terser.status);
+    }
+
+    fs.writeFileSync(WASM_BINDING_THREADED_MIN_WORKER_JS_PATH, terser.stdout);
+    fs.writeFileSync(WASM_THREADED_WORKER_JS_PATH, COPYRIGHT_BANNER + terser.stdout);
+
+    validateFile(WASM_BINDING_THREADED_MIN_WORKER_JS_PATH);
+    validateFile(WASM_THREADED_WORKER_JS_PATH);
+  } catch (e) {
+    npmlog.error('Build', `Failed to run terser on ort-wasm-threaded.worker.js. ERR: ${e}`);
+    throw e;
+  }
+  npmlog.info('Build', 'Minimizing file "ort-wasm-threaded.worker.js"... DONE');
 }
 
 npmlog.info('Build', 'Building bundle...');
 {
-  npmlog.info('Build.Bundle', '(1/2) Retrieving npm bin folder...');
-  const npmBin = execSync('npm bin', {encoding: 'utf8'}).trimRight();
-  npmlog.info('Build.Bundle', `(1/2) Retrieving npm bin folder... DONE, folder: ${npmBin}`);
-
-  npmlog.info('Build.Bundle', '(2/2) Running webpack to generate bundles...');
+  npmlog.info('Build.Bundle', 'Running webpack to generate bundles...');
   const webpackCommand = path.join(npmBin, 'webpack');
   const webpackArgs = ['--env', `--bundle-mode=${MODE}`];
   npmlog.info('Build.Bundle', `CMD: ${webpackCommand} ${webpackArgs.join(' ')}`);
@@ -71,6 +135,6 @@ npmlog.info('Build', 'Building bundle...');
     console.error(webpack.error);
     process.exit(webpack.status === null ? undefined : webpack.status);
   }
-  npmlog.info('Build.Bundle', '(2/2) Running webpack to generate bundles... DONE');
+  npmlog.info('Build.Bundle', 'Running webpack to generate bundles... DONE');
 }
 npmlog.info('Build', 'Building bundle... DONE');
diff --git a/js/web/script/test-runner-cli-args.ts b/js/web/script/test-runner-cli-args.ts
index b2519e8617..b83b58a30f 100644
--- a/js/web/script/test-runner-cli-args.ts
+++ b/js/web/script/test-runner-cli-args.ts
@@ -119,11 +119,12 @@ export interface TestRunnerCliArgs {
    *
    * For running tests, the default mode is 'dev'. If flag '--perf' is set, the mode will be set to 'perf'.
    *
-   * Mode   | Output File        | Main                 | Source Map         | Webpack Config
-   * ------ | ------------------ | -------------------- | ------------------ | --------------
-   * prod   | /dist/ort.min.js   | /lib/index.ts        | source-map         | production
-   * dev    | /test/ort.dev.js   | /test/test-main.ts   | inline-source-map  | development
-   * perf   | /test/ort.perf.js  | /test/test-main.ts   | (none)             | production
+   * Mode   | Output File           | Main                 | Source Map         | Webpack Config
+   * ------ | --------------------- | -------------------- | ------------------ | --------------
+   * prod   | /dist/ort.min.js      | /lib/index.ts        | source-map         | production
+   * node   | /dist/ort-web.node.js | /lib/index.ts        | source-map         | production
+   * dev    | /test/ort.dev.js      | /test/test-main.ts   | inline-source-map  | development
+   * perf   | /test/ort.perf.js     | /test/test-main.ts   | (none)             | production
    */
   bundleMode: TestRunnerCliArgs.BundleMode;
 
@@ -298,24 +299,23 @@ export function parseTestRunnerCliArgs(cmdlineArgs: string[]): TestRunnerCliArgs
 
   const mode = args._.length === 0 ? 'suite0' : args._[0];
 
-  // Option: -b=<...>, --backend=<...>
-  const backendArgs = args.backend || args.b;
-  const backend = (typeof backendArgs !== 'string') ? ['webgl', 'wasm'] : backendArgs.split(',');
-  for (const b of backend) {
-    if (b !== 'webgl' && b !== 'wasm') {
-      throw new Error(`not supported backend ${b}`);
-    }
-  }
-
   // Option: -e=<...>, --env=<...>
   const envArg = args.env || args.e;
   const env = (typeof envArg !== 'string') ? 'chrome' : envArg;
   if (['chrome', 'edge', 'firefox', 'electron', 'safari', 'node', 'bs'].indexOf(env) === -1) {
     throw new Error(`not supported env ${env}`);
   }
-  if (env === 'node') {
-    // TODO: support node
-    throw new Error('node is currently not supported.');
+
+  // Option: -b=<...>, --backend=<...>
+  const browserBackends = ['webgl', 'wasm'];
+  const nodejsBackends = ['cpu', 'wasm'];
+  const backendArgs = args.backend || args.b;
+  const backend =
+      (typeof backendArgs !== 'string') ? (env === 'node' ? nodejsBackends : browserBackends) : backendArgs.split(',');
+  for (const b of backend) {
+    if ((env !== 'node' && browserBackends.indexOf(b) === -1) || (env === 'node' && nodejsBackends.indexOf(b) === -1)) {
+      throw new Error(`backend ${b} is not supported in env ${env}`);
+    }
   }
 
   // Options:
diff --git a/js/web/script/test-runner-cli.ts b/js/web/script/test-runner-cli.ts
index d657e390ac..95bad0fe3b 100644
--- a/js/web/script/test-runner-cli.ts
+++ b/js/web/script/test-runner-cli.ts
@@ -5,7 +5,7 @@
 /* eslint-disable @typescript-eslint/no-use-before-define */
 
 import {execSync, spawnSync} from 'child_process';
-import * as fs from 'fs';
+import * as fs from 'fs-extra';
 import * as globby from 'globby';
 import {default as minimatch} from 'minimatch';
 import npmlog from 'npmlog';
@@ -399,31 +399,45 @@ function run(config: Test.Config) {
       `(1/5) Writing file cache to file: testdata-file-cache-*.json ... ${
           fileCacheUrls.length > 0 ? `DONE, ${fileCacheUrls.length} file(s) generated` : 'SKIPPED'}`);
 
-  // STEP 2. write the config to testdata-config.js
-  npmlog.info('TestRunnerCli.Run', '(2/5) Writing config to file: testdata-config.js ...');
+  // STEP 2. write the config to testdata-config.json
+  npmlog.info('TestRunnerCli.Run', '(2/5) Writing config to file: testdata-config.json ...');
   saveConfig(config);
-  npmlog.info('TestRunnerCli.Run', '(2/5) Writing config to file: testdata-config.js ... DONE');
+  npmlog.info('TestRunnerCli.Run', '(2/5) Writing config to file: testdata-config.json ... DONE');
 
   // STEP 3. get npm bin folder
   npmlog.info('TestRunnerCli.Run', '(3/5) Retrieving npm bin folder...');
   const npmBin = execSync('npm bin', {encoding: 'utf8'}).trimRight();
   npmlog.info('TestRunnerCli.Run', `(3/5) Retrieving npm bin folder... DONE, folder: ${npmBin}`);
 
+  // STEP 4. generate bundle
+  npmlog.info('TestRunnerCli.Run', '(4/5) Running build to generate bundle...');
+  const buildCommand = `node ${path.join(__dirname, 'build')}`;
+  const buildArgs = [`--bundle-mode=${args.env === 'node' ? 'node' : args.bundleMode}`];
+  if (args.backends.indexOf('wasm') === -1) {
+    buildArgs.push('--no-wasm');
+  }
+  npmlog.info('TestRunnerCli.Run', `CMD: ${buildCommand} ${buildArgs.join(' ')}`);
+  const build = spawnSync(buildCommand, buildArgs, {shell: true, stdio: 'inherit'});
+  if (build.status !== 0) {
+    console.error(build.error);
+    process.exit(build.status === null ? undefined : build.status);
+  }
+  npmlog.info('TestRunnerCli.Run', '(4/5) Running build to generate bundle... DONE');
+
   if (args.env === 'node') {
-    // STEP 4. use tsc to build ONNX Runtime Web
-    npmlog.info('TestRunnerCli.Run', '(4/5) Running tsc...');
+    // STEP 5. run tsc and run mocha
+    npmlog.info('TestRunnerCli.Run', '(5/5) Running tsc...');
     const tscCommand = path.join(npmBin, 'tsc');
     const tsc = spawnSync(tscCommand, {shell: true, stdio: 'inherit'});
     if (tsc.status !== 0) {
       console.error(tsc.error);
       process.exit(tsc.status === null ? undefined : tsc.status);
     }
-    npmlog.info('TestRunnerCli.Run', '(4/5) Running tsc... DONE');
+    npmlog.info('TestRunnerCli.Run', '(5/5) Running tsc... DONE');
 
-    // STEP 5. run mocha
     npmlog.info('TestRunnerCli.Run', '(5/5) Running mocha...');
     const mochaCommand = path.join(npmBin, 'mocha');
-    const mochaArgs = [path.join(TEST_ROOT, 'test-main'), '--timeout 60000'];
+    const mochaArgs = [path.join(TEST_ROOT, 'test-main'), `--timeout ${args.debug ? 9999999 : 60000}`];
     npmlog.info('TestRunnerCli.Run', `CMD: ${mochaCommand} ${mochaArgs.join(' ')}`);
     const mocha = spawnSync(mochaCommand, mochaArgs, {shell: true, stdio: 'inherit'});
     if (mocha.status !== 0) {
@@ -433,21 +447,6 @@ function run(config: Test.Config) {
     npmlog.info('TestRunnerCli.Run', '(5/5) Running mocha... DONE');
 
   } else {
-    // STEP 4. generate bundle
-    npmlog.info('TestRunnerCli.Run', '(4/5) Running build to generate bundle...');
-    const buildCommand = `node ${path.join(__dirname, 'build')}`;
-    const buildArgs = [`--bundle-mode=${args.bundleMode}`];
-    if (args.backends.indexOf('wasm') === -1) {
-      buildArgs.push('--no-wasm');
-    }
-    npmlog.info('TestRunnerCli.Run', `CMD: ${buildCommand} ${buildArgs.join(' ')}`);
-    const build = spawnSync(buildCommand, buildArgs, {shell: true, stdio: 'inherit'});
-    if (build.status !== 0) {
-      console.error(build.error);
-      process.exit(build.status === null ? undefined : build.status);
-    }
-    npmlog.info('TestRunnerCli.Run', '(4/5) Running build to generate bundle... DONE');
-
     // STEP 5. use Karma to run test
     npmlog.info('TestRunnerCli.Run', '(5/5) Running karma to start test runner...');
     const karmaCommand = path.join(npmBin, 'karma');
@@ -549,39 +548,7 @@ function saveOneFileCache(index: number, fileCache: Test.FileCache) {
 }
 
 function saveConfig(config: Test.Config) {
-  let setOptions = '';
-  if (config.options.debug !== undefined) {
-    setOptions += `ort.env.debug = ${config.options.debug};`;
-  }
-  if (config.options.webglFlags && config.options.webglFlags.contextId !== undefined) {
-    setOptions += `ort.env.webgl.contextId = ${JSON.stringify(config.options.webglFlags.contextId)};`;
-  }
-  if (config.options.webglFlags && config.options.webglFlags.matmulMaxBatchSize !== undefined) {
-    setOptions += `ort.env.webgl.matmulMaxBatchSize = ${config.options.webglFlags.matmulMaxBatchSize};`;
-  }
-  if (config.options.webglFlags && config.options.webglFlags.textureCacheMode !== undefined) {
-    setOptions += `ort.env.webgl.textureCacheMode = ${JSON.stringify(config.options.webglFlags.textureCacheMode)};`;
-  }
-  if (config.options.webglFlags && config.options.webglFlags.pack !== undefined) {
-    setOptions += `ort.env.webgl.pack = ${JSON.stringify(config.options.webglFlags.pack)};`;
-  }
-  if (config.options.wasmFlags && config.options.wasmFlags.numThreads !== undefined) {
-    setOptions += `ort.env.wasm.numThreads = ${JSON.stringify(config.options.wasmFlags.numThreads)};`;
-  }
-  if (config.options.wasmFlags && config.options.wasmFlags.loggingLevel !== undefined) {
-    setOptions += `ort.env.wasm.loggingLevel = ${JSON.stringify(config.options.wasmFlags.loggingLevel)};`;
-  }
-  if (config.options.wasmFlags && config.options.wasmFlags.initTimeout !== undefined) {
-    setOptions += `ort.env.wasm.initTimeout = ${JSON.stringify(config.options.wasmFlags.initTimeout)};`;
-  }
-  // TODO: support onnxruntime nodejs binding
-  // if (config.model.some(testGroup => testGroup.tests.some(test => test.backend === 'cpu'))) {
-  //   setOptions += 'require(\'onnxruntime-node\');';
-  // }
-
-  fs.writeFileSync(path.join(TEST_ROOT, './testdata-config.js'), `${setOptions}
-
-ort.env.ORT_WEB_TEST_DATA=${JSON.stringify(config)};`);
+  fs.writeJSONSync(path.join(TEST_ROOT, './testdata-config.json'), config);
 }
 
 function getBrowserNameFromEnv(env: TestRunnerCliArgs['env'], debug?: boolean) {
diff --git a/js/web/test/e2e/.gitignore b/js/web/test/e2e/.gitignore
new file mode 100644
index 0000000000..c25f6c22a9
--- /dev/null
+++ b/js/web/test/e2e/.gitignore
@@ -0,0 +1 @@
+!**/*.js
diff --git a/js/web/test/e2e/browser-test-wasm-no-threads.js b/js/web/test/e2e/browser-test-wasm-no-threads.js
new file mode 100644
index 0000000000..6ed7193b40
--- /dev/null
+++ b/js/web/test/e2e/browser-test-wasm-no-threads.js
@@ -0,0 +1,7 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+it('Browser E2E testing - WebAssembly backend (no threads)', async function () {
+  ort.env.wasm.numThreads = 1;
+  await testFunction(ort, { executionProviders: ['wasm'] });
+});
diff --git a/js/web/test/e2e/browser-test-wasm.js b/js/web/test/e2e/browser-test-wasm.js
new file mode 100644
index 0000000000..9d91aed76d
--- /dev/null
+++ b/js/web/test/e2e/browser-test-wasm.js
@@ -0,0 +1,6 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+it('Browser E2E testing - WebAssembly backend', async function () {
+  await testFunction(ort, { executionProviders: ['wasm'] });
+});
diff --git a/js/web/test/e2e/browser-test-webgl.js b/js/web/test/e2e/browser-test-webgl.js
new file mode 100644
index 0000000000..4da09438df
--- /dev/null
+++ b/js/web/test/e2e/browser-test-webgl.js
@@ -0,0 +1,6 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+it('Browser E2E testing - WebGL backend', async function () {
+  await testFunction(ort, { executionProviders: ['webgl'] });
+});
diff --git a/js/web/test/e2e/common.js b/js/web/test/e2e/common.js
new file mode 100644
index 0000000000..e7b9b22ef1
--- /dev/null
+++ b/js/web/test/e2e/common.js
@@ -0,0 +1,36 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+function assert(cond) {
+  if (!cond) throw new Error();
+}
+
+var testFunction = async function (ort, options) {
+  const session = await ort.InferenceSession.create('./model.onnx', options || {});
+
+  const dataA = Float32Array.from([1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12]);
+  const dataB = Float32Array.from([10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120]);
+
+  const fetches = await session.run({
+    a: new ort.Tensor('float32', dataA, [3, 4]),
+    b: new ort.Tensor('float32', dataB, [4, 3])
+  });
+
+  const c = fetches.c;
+
+  assert(c instanceof ort.Tensor);
+  assert(c.dims.length === 2 && c.dims[0] === 3 && c.dims[1] === 3);
+  assert(c.data[0] === 700);
+  assert(c.data[1] === 800);
+  assert(c.data[2] === 900);
+  assert(c.data[3] === 1580);
+  assert(c.data[4] === 1840);
+  assert(c.data[5] === 2100);
+  assert(c.data[6] === 2460);
+  assert(c.data[7] === 2880);
+  assert(c.data[8] === 3300);
+};
+
+if (typeof module === 'object') {
+  module.exports = testFunction;
+}
diff --git a/js/web/test/e2e/karma.conf.js b/js/web/test/e2e/karma.conf.js
new file mode 100644
index 0000000000..a0552c0f09
--- /dev/null
+++ b/js/web/test/e2e/karma.conf.js
@@ -0,0 +1,51 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+const args = require('minimist')(process.argv.slice(2));
+const SELF_HOST = !!args['self-host'];
+const TEST_MAIN = args['test-main'];
+if (typeof TEST_MAIN !== 'string') {
+  throw new Error('flag --test-main=<TEST_MAIN_JS_FILE> is required');
+}
+const USER_DATA = args['user-data'];
+if (typeof USER_DATA !== 'string') {
+  throw new Error('flag --user-data=<CHROME_USER_DATA_FOLDER> is required');
+}
+
+module.exports = function (config) {
+  const distPrefix = SELF_HOST ? './node_modules/onnxruntime-web/dist/' : 'http://localhost:8081/dist/';
+  config.set({
+    frameworks: ['mocha'],
+    files: [
+      { pattern: distPrefix + 'ort.js' },
+      { pattern: './common.js' },
+      { pattern: TEST_MAIN },
+      { pattern: './node_modules/onnxruntime-web/dist/**/*', included: false, nocache: true },
+      { pattern: './model.onnx', included: false }
+    ],
+    proxies: {
+      '/model.onnx': '/base/model.onnx',
+    },
+    client: { captureConsole: true, mocha: { expose: ['body'], timeout: 60000 } },
+    reporters: ['mocha'],
+    captureTimeout: 120000,
+    reportSlowerThan: 100,
+    browserDisconnectTimeout: 600000,
+    browserNoActivityTimeout: 300000,
+    browserDisconnectTolerance: 0,
+    browserSocketTimeout: 60000,
+    hostname: 'localhost',
+    browsers: [],
+    customLaunchers: {
+      Chrome_default: {
+        base: 'Chrome',
+        chromeDataDir: USER_DATA
+      },
+      Chrome_no_threads: {
+        base: 'Chrome',
+        chromeDataDir: USER_DATA,
+        // TODO: no-thread flags
+      }
+    }
+  });
+};
diff --git a/js/web/test/e2e/model.onnx b/js/web/test/e2e/model.onnx
new file mode 100644
index 0000000000..088124bd48
--- /dev/null
+++ b/js/web/test/e2e/model.onnx
@@ -0,0 +1,16 @@
+backend-test:b
+
+
a
+
b
c"MatMultest_matmul_2dZ
+
a
+

+
+Z
+
b
+

+
+b
+
c
+

+
+B	
\ No newline at end of file
diff --git a/js/web/test/e2e/node-test-main-no-threads.js b/js/web/test/e2e/node-test-main-no-threads.js
new file mode 100644
index 0000000000..2eb7ee25a3
--- /dev/null
+++ b/js/web/test/e2e/node-test-main-no-threads.js
@@ -0,0 +1,10 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+const ort = require('onnxruntime-web');
+const testFunction = require('./common');
+
+it('Browser E2E testing - WebAssembly backend', async function () {
+  ort.env.wasm.numThreads = 1;
+  await testFunction(ort, { executionProviders: ['wasm'] });
+});
diff --git a/js/web/test/e2e/node-test-main.js b/js/web/test/e2e/node-test-main.js
new file mode 100644
index 0000000000..bbac8e478b
--- /dev/null
+++ b/js/web/test/e2e/node-test-main.js
@@ -0,0 +1,11 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+const ort = require('onnxruntime-web');
+const testFunction = require('./common');
+
+it('Browser E2E testing - WebAssembly backend', async function () {
+  await testFunction(ort, { executionProviders: ['wasm'] });
+
+  process.exit();
+});
diff --git a/js/web/test/e2e/package.json b/js/web/test/e2e/package.json
new file mode 100644
index 0000000000..7e0c78e8ff
--- /dev/null
+++ b/js/web/test/e2e/package.json
@@ -0,0 +1,13 @@
+{
+  "devDependencies": {
+    "fs-extra": "^9.1.0",
+    "globby": "^11.0.3",
+    "karma": "^6.3.2",
+    "karma-chrome-launcher": "^3.1.0",
+    "karma-mocha": "^2.0.1",
+    "karma-mocha-reporter": "^2.2.5",
+    "light-server": "^2.9.1",
+    "minimist": "^1.2.5",
+    "mocha": "^8.3.2"
+  }
+}
diff --git a/js/web/test/e2e/run.js b/js/web/test/e2e/run.js
new file mode 100644
index 0000000000..02cb3a3326
--- /dev/null
+++ b/js/web/test/e2e/run.js
@@ -0,0 +1,109 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+const path = require('path');
+const fs = require('fs-extra');
+const globby = require('globby');
+const { spawn } = require('child_process');
+const startServer = require('./simple-http-server');
+
+// copy whole folder to out-side of <ORT_ROOT>/js/ because we need to test in a folder that no `package.json` file
+// exists in its parent folder.
+// here we use <ORT_ROOT>/build/js/e2e/ for the test
+
+const TEST_E2E_SRC_FOLDER = __dirname;
+const JS_ROOT_FOLDER = path.resolve(__dirname, '../../..');
+const TEST_E2E_RUN_FOLDER = path.resolve(JS_ROOT_FOLDER, '../build/js/e2e');
+const NPM_CACHE_FOLDER = path.resolve(TEST_E2E_RUN_FOLDER, '../npm_cache');
+const CHROME_USER_DATA_FOLDER = path.resolve(TEST_E2E_RUN_FOLDER, '../user_data');
+fs.emptyDirSync(TEST_E2E_RUN_FOLDER);
+fs.emptyDirSync(NPM_CACHE_FOLDER);
+fs.emptyDirSync(CHROME_USER_DATA_FOLDER);
+fs.copySync(TEST_E2E_SRC_FOLDER, TEST_E2E_RUN_FOLDER);
+
+// find packed package
+
+const ORT_COMMON_FOLDER = path.resolve(JS_ROOT_FOLDER, 'common');
+const ORT_COMMON_PACKED_FILEPATH_CANDIDATES = globby.sync('onnxruntime-common-*.tgz', { cwd: ORT_COMMON_FOLDER });
+if (ORT_COMMON_PACKED_FILEPATH_CANDIDATES.length !== 1) {
+  throw new Error('cannot find exactly single package for onnxruntime-common.');
+}
+const ORT_COMMON_PACKED_FILEPATH = path.resolve(ORT_COMMON_FOLDER, ORT_COMMON_PACKED_FILEPATH_CANDIDATES[0]);
+
+const ORT_WEB_FOLDER = path.resolve(JS_ROOT_FOLDER, 'web');
+const ORT_WEB_PACKED_FILEPATH_CANDIDATES = globby.sync('onnxruntime-web-*.tgz', { cwd: ORT_WEB_FOLDER });
+if (ORT_WEB_PACKED_FILEPATH_CANDIDATES.length !== 1) {
+  throw new Error('cannot find exactly single package for onnxruntime-web.');
+}
+const ORT_WEB_PACKED_FILEPATH = path.resolve(ORT_WEB_FOLDER, ORT_WEB_PACKED_FILEPATH_CANDIDATES[0]);
+
+// we start here:
+
+async function main() {
+  // install dev dependencies
+  await runInShell(`npm install"`);
+
+  // npm install with "--cache" to install packed packages with an empty cache folder
+  await runInShell(`npm install --cache "${NPM_CACHE_FOLDER}" "${ORT_COMMON_PACKED_FILEPATH}" "${ORT_WEB_PACKED_FILEPATH}"`);
+
+  // test case run in Node.js
+  await testAllNodejsCases();
+
+  // test cases with self-host (ort hosted in same origin)
+  await testAllBrowserCases({ hostInKarma: true });
+
+  // test cases without self-host (ort hosted in same origin)
+  startServer(path.resolve(TEST_E2E_RUN_FOLDER, 'node_modules', 'onnxruntime-web'));
+  await testAllBrowserCases({ hostInKarma: false });
+
+  // no error occurs, exit with code 0
+  process.exit(0);
+}
+
+async function testAllNodejsCases() {
+  await runInShell('node ./node_modules/mocha/bin/mocha ./node-test-main-no-threads.js');
+  await runInShell('node ./node_modules/mocha/bin/mocha ./node-test-main.js');
+  await runInShell('node --experimental-wasm-threads --experimental-wasm-bulk-memory ./node_modules/mocha/bin/mocha ./node-test-main-no-threads.js');
+  await runInShell('node --experimental-wasm-threads --experimental-wasm-bulk-memory ./node_modules/mocha/bin/mocha ./node-test-main.js');
+}
+
+async function testAllBrowserCases({ hostInKarma }) {
+  await runKarma({ hostInKarma, main: './browser-test-webgl.js', browser: 'Chrome_default' });
+  await runKarma({ hostInKarma, main: './browser-test-wasm.js', browser: 'Chrome_default' });
+  await runKarma({ hostInKarma, main: './browser-test-wasm-no-threads.js', browser: 'Chrome_default' });
+}
+
+async function runKarma({ hostInKarma, main, browser }) {
+  const selfHostFlag = hostInKarma ? '--self-host' : '';
+  await runInShell(
+    `npx karma start --single-run --browsers ${browser} ${selfHostFlag} --test-main=${main} --user-data=${CHROME_USER_DATA_FOLDER}`);
+}
+
+async function runInShell(cmd) {
+  console.log('===============================================================');
+  console.log(' Running command in shell:');
+  console.log(' > ' + cmd);
+  console.log('===============================================================');
+  let complete = false;
+  const childProcess = spawn(cmd, { shell: true, stdio: 'inherit', cwd: TEST_E2E_RUN_FOLDER });
+  childProcess.on('close', function (code) {
+    if (code !== 0) {
+      process.exit(code);
+    } else {
+      complete = true;
+    }
+  });
+  while (!complete) {
+    await delay(100);
+  }
+}
+
+async function delay(ms) {
+  return new Promise(function (resolve) {
+    setTimeout(function () {
+      resolve();
+    }, ms);
+  });
+}
+
+main();
diff --git a/js/web/test/e2e/simple-http-server.js b/js/web/test/e2e/simple-http-server.js
new file mode 100644
index 0000000000..f5e96e8aa0
--- /dev/null
+++ b/js/web/test/e2e/simple-http-server.js
@@ -0,0 +1,58 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+// this is a simple HTTP server that enables CORS.
+// following code is based on https://developer.mozilla.org/en-US/docs/Learn/Server-side/Node_server_without_framework
+
+var http = require('http');
+var fs = require('fs');
+var path = require('path');
+
+module.exports = function (dir) {
+  http.createServer(function (request, response) {
+    console.log('request ', request.url);
+
+    var filePath = '.' + request.url;
+
+    var extname = String(path.extname(filePath)).toLowerCase();
+    var mimeTypes = {
+      '.html': 'text/html',
+      '.js': 'text/javascript',
+      '.css': 'text/css',
+      '.json': 'application/json',
+      '.png': 'image/png',
+      '.jpg': 'image/jpg',
+      '.gif': 'image/gif',
+      '.svg': 'image/svg+xml',
+      '.wav': 'audio/wav',
+      '.mp4': 'video/mp4',
+      '.woff': 'application/font-woff',
+      '.ttf': 'application/font-ttf',
+      '.eot': 'application/vnd.ms-fontobject',
+      '.otf': 'application/font-otf',
+      '.wasm': 'application/wasm'
+    };
+
+    var contentType = mimeTypes[extname] || 'application/octet-stream';
+
+    fs.readFile(path.resolve(dir, filePath), function (error, content) {
+      if (error) {
+        if (error.code == 'ENOENT') {
+          response.writeHead(404);
+          response.end('404');
+        }
+        else {
+          response.writeHead(500);
+          response.end('500');
+        }
+      }
+      else {
+        response.setHeader('access-control-allow-origin', '*');
+        response.writeHead(200, { 'Content-Type': contentType });
+        response.end(content, 'utf-8');
+      }
+    });
+
+  }).listen(8081);
+  console.log('Server running at http://127.0.0.1:8081/');
+};
diff --git a/js/web/test/test-main.ts b/js/web/test/test-main.ts
index 86b2dce371..c68dc3f272 100644
--- a/js/web/test/test-main.ts
+++ b/js/web/test/test-main.ts
@@ -1,16 +1,48 @@
 // Copyright (c) Microsoft Corporation. All rights reserved.
 // Licensed under the MIT License.
 
-import '../lib/index'; // this need to be the first line
+// Load onnxruntime-web and testdata-config.
+// NOTE: this need to be called before import any other library.
+const ort = require('..');
+const ORT_WEB_TEST_CONFIG = require('./testdata-config.json') as Test.Config;
 
-import * as ort from 'onnxruntime-common';
 import * as platform from 'platform';
 
 import {Logger} from '../lib/onnxjs/instrument';
 
 import {Test} from './test-types';
 
-const ORT_WEB_TEST_CONFIG = (ort.env as any).ORT_WEB_TEST_DATA as Test.Config;
+if (ORT_WEB_TEST_CONFIG.model.some(testGroup => testGroup.tests.some(test => test.backend === 'cpu'))) {
+  // require onnxruntime-node
+  require('../../node');
+}
+
+// set flags
+const options = ORT_WEB_TEST_CONFIG.options;
+if (options.debug !== undefined) {
+  ort.env.debug = options.debug;
+}
+if (ort.env.webgl && options.webglFlags && options.webglFlags.contextId !== undefined) {
+  ort.env.webgl.contextId = options.webglFlags.contextId;
+}
+if (ort.env.webgl && options.webglFlags && options.webglFlags.matmulMaxBatchSize !== undefined) {
+  ort.env.webgl.matmulMaxBatchSize = options.webglFlags.matmulMaxBatchSize;
+}
+if (ort.env.webgl && options.webglFlags && options.webglFlags.textureCacheMode !== undefined) {
+  ort.env.webgl.textureCacheMode = options.webglFlags.textureCacheMode;
+}
+if (ort.env.webgl && options.webglFlags && options.webglFlags.pack !== undefined) {
+  ort.env.webgl.pack = options.webglFlags.pack;
+}
+if (ort.env.wasm && options.wasmFlags && options.wasmFlags.numThreads !== undefined) {
+  ort.env.wasm.numThreads = options.wasmFlags.numThreads;
+}
+if (ort.env.wasm && options.wasmFlags && options.wasmFlags.loggingLevel !== undefined) {
+  ort.env.wasm.loggingLevel = options.wasmFlags.loggingLevel;
+}
+if (ort.env.wasm && options.wasmFlags && options.wasmFlags.initTimeout !== undefined) {
+  ort.env.wasm.initTimeout = options.wasmFlags.initTimeout;
+}
 
 // Set logging configuration
 for (const logConfig of ORT_WEB_TEST_CONFIG.log) {
diff --git a/js/web/test/test-suite-whitelist.jsonc b/js/web/test/test-suite-whitelist.jsonc
index 4d6efebc42..8539d6d150 100644
--- a/js/web/test/test-suite-whitelist.jsonc
+++ b/js/web/test/test-suite-whitelist.jsonc
@@ -1,4 +1,9 @@
 {
+  "cpu": {
+    "onnx": [],
+    "node": [],
+    "ops": []
+  },
   "webgl": {
     "onnx": ["resnet50", "squeezenet", "tiny_yolov2", "emotion_ferplus"],
     "node": [
diff --git a/js/web/webpack.config.js b/js/web/webpack.config.js
index 768b75ff4b..18160af8e3 100644
--- a/js/web/webpack.config.js
+++ b/js/web/webpack.config.js
@@ -7,38 +7,33 @@ const NodePolyfillPlugin = require('node-polyfill-webpack-plugin');
 const TerserPlugin = require("terser-webpack-plugin");
 const minimist = require('minimist');
 
-function addCopyrightBannerPlugin(mode) {
-  const VERSION = require(path.join(__dirname, 'package.json')).version;
-  const COPYRIGHT_BANNER = `/*!
- * ONNX Runtime Web v${VERSION}
- * Copyright (c) Microsoft Corporation. All rights reserved.
- * Licensed under the MIT License.
- */`;
+const VERSION = require(path.join(__dirname, 'package.json')).version;
+const COPYRIGHT_BANNER = `/*!
+* ONNX Runtime Web v${VERSION}
+* Copyright (c) Microsoft Corporation. All rights reserved.
+* Licensed under the MIT License.
+*/`;
 
-  if (mode === 'production') {
-    return new TerserPlugin({
-      extractComments: false,
-      terserOptions: {
-        format: {
-          preamble: COPYRIGHT_BANNER,
-          comments: false,
-        },
-        compress: {
-          passes: 2
-        },
-        mangle: {
-          reserved: ["_scriptDir"]
-        }
+function defaultTerserPluginOptions() {
+  return {
+    extractComments: false,
+    terserOptions: {
+      format: {
+        comments: false,
+      },
+      compress: {
+        passes: 2
+      },
+      mangle: {
+        reserved: ["_scriptDir"]
       }
-    });
-  } else {
-    return new webpack.BannerPlugin({ banner: COPYRIGHT_BANNER, raw: true });
-  }
+    }
+  };
 }
 
 // common config for release bundle
 function buildConfig({ filename, format, target, mode, devtool }) {
-  return {
+  const config = {
     target: [format === 'commonjs' ? 'node' : 'web', target],
     entry: path.resolve(__dirname, 'lib/index.ts'),
     output: {
@@ -49,10 +44,7 @@ function buildConfig({ filename, format, target, mode, devtool }) {
       }
     },
     resolve: { extensions: ['.ts', '.js'] },
-    plugins: [
-      new webpack.WatchIgnorePlugin({ paths: [/\.js$/, /\.d\.ts$/] }),
-      addCopyrightBannerPlugin(mode),
-    ],
+    plugins: [new webpack.WatchIgnorePlugin({ paths: [/\.js$/, /\.d\.ts$/] })],
     module: {
       rules: [{
         test: /\.ts$/,
@@ -64,11 +56,28 @@ function buildConfig({ filename, format, target, mode, devtool }) {
             }
           }
         ]
+      }, {
+        test: /\.worker.js$/,
+        type: 'asset/source'
       }]
     },
     mode,
     devtool
   };
+
+  if (mode === 'production') {
+    config.resolve.alias = {
+      './binding/ort-wasm-threaded.js': './binding/ort-wasm-threaded.min.js',
+      './binding/ort-wasm-threaded.worker.js': './binding/ort-wasm-threaded.min.worker.js'
+    };
+    const options = defaultTerserPluginOptions();
+    options.terserOptions.format.preamble = COPYRIGHT_BANNER;
+    config.plugins.push(new TerserPlugin(options));
+  } else {
+    config.plugins.push(new webpack.BannerPlugin({ banner: COPYRIGHT_BANNER, raw: true }));
+  }
+
+  return config;
 }
 
 // "ort{.min}.js" config
@@ -108,10 +117,9 @@ function buildOrtWebConfig({
     config.externals.path = 'path';
     config.externals.fs = 'fs';
     config.externals.util = 'util';
-  }
-  // in browser, do not use those node builtin modules
-  if (format === 'umd') {
-    config.resolve.fallback = { path: false, fs: false, util: false };
+    config.externals.worker_threads = 'worker_threads';
+    config.externals.perf_hooks = 'perf_hooks';
+    config.externals.os = 'os';
   }
   return config;
 }
@@ -123,7 +131,7 @@ function buildTestRunnerConfig({
   mode = 'production',
   devtool = 'source-map'
 }) {
-  return {
+  const config = {
     target: ['web', target],
     entry: path.resolve(__dirname, 'test/test-main.ts'),
     output: {
@@ -139,6 +147,7 @@ function buildTestRunnerConfig({
       'fs': 'fs',
       'perf_hooks': 'perf_hooks',
       'worker_threads': 'worker_threads',
+      '../../node': '../../node'
     },
     resolve: {
       extensions: ['.ts', '.js'],
@@ -148,7 +157,6 @@ function buildTestRunnerConfig({
     plugins: [
       new webpack.WatchIgnorePlugin({ paths: [/\.js$/, /\.d\.ts$/] }),
       new NodePolyfillPlugin(),
-      addCopyrightBannerPlugin(mode),
     ],
     module: {
       rules: [{
@@ -161,16 +169,25 @@ function buildTestRunnerConfig({
             }
           }
         ]
+      }, {
+        test: /\.worker\.js$/,
+        type: 'asset/source'
       }]
     },
     mode: mode,
     devtool: devtool,
   };
+
+  if (mode === 'production') {
+    config.plugins.push(new TerserPlugin(defaultTerserPluginOptions()));
+  }
+
+  return config;
 }
 
 module.exports = () => {
   const args = minimist(process.argv);
-  const bundleMode = args['bundle-mode'] || 'prod';  // 'prod'|'dev'|'perf'|undefined;
+  const bundleMode = args['bundle-mode'] || 'prod';  // 'prod'|'dev'|'perf'|'node'|undefined;
   const builds = [];
 
   switch (bundleMode) {
@@ -193,7 +210,10 @@ module.exports = () => {
         buildOrtWebConfig({ suffix: '.es6.min', target: 'es6' }),
         // ort-web.es6.js
         buildOrtWebConfig({ suffix: '.es6', mode: 'development', devtool: 'inline-source-map', target: 'es6' }),
+      );
 
+    case 'node':
+      builds.push(
         // ort-web.node.js
         buildOrtWebConfig({ suffix: '.node', format: 'commonjs' }),
       );
diff --git a/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml b/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml
index 83fca75cae..d5a090db2f 100644
--- a/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml
+++ b/tools/ci_build/github/azure-pipelines/win-wasm-ci-pipeline.yml
@@ -157,7 +157,6 @@ jobs:
       sourceFolder: $(Pipeline.Workspace)\artifacts
       contents: |
         **\*.wasm
-        **\*.worker.js
       targetFolder: $(Build.SourcesDirectory)\js\web\dist
       flattenFolders: true
     displayName: 'Binplace dist files'
@@ -166,7 +165,6 @@ jobs:
       sourceFolder: $(Pipeline.Workspace)\artifacts
       contents: |
         **\*.js
-        !**\*.worker.js
       targetFolder: $(Build.SourcesDirectory)\js\web\lib\wasm\binding
       flattenFolders: true
     displayName: 'Binplace js files'
@@ -212,6 +210,11 @@ jobs:
     workingDirectory: '$(Build.SourcesDirectory)\js\web'
     displayName: 'Generate NPM package (onnxruntime-web)'
     condition: and(succeeded(), eq(variables['BuildConfig'], 'Release'))
+  - script: |
+      npm run test:e2e
+    workingDirectory: '$(Build.SourcesDirectory)\js\web'
+    displayName: 'E2E package consuming test'
+    condition: and(succeeded(), eq(variables['BuildConfig'], 'Release'))
   - task: CopyFiles@2
     inputs:
       sourceFolder: $(Build.SourcesDirectory)\js\common