diff --git a/onnxruntime/core/providers/webnn/builders/helper.h b/onnxruntime/core/providers/webnn/builders/helper.h
index 28857d3002..8b8b85339a 100644
--- a/onnxruntime/core/providers/webnn/builders/helper.h
+++ b/onnxruntime/core/providers/webnn/builders/helper.h
@@ -179,7 +179,7 @@ static const InlinedHashMap<std::string, WebnnOpInfo> op_map = {
     {"Min", {"min", true}},
     {"Mul", {"mul", true}},
     {"Neg", {"neg", true}},
-    {"Not", {"not", false}},
+    {"Not", {"logicalNot", false}},
     {"Pad", {"pad", true}},
     {"Pow", {"pow", false}},
     {"PRelu", {"prelu", true}},
diff --git a/onnxruntime/core/providers/webnn/builders/impl/unary_op_builder.cc b/onnxruntime/core/providers/webnn/builders/impl/unary_op_builder.cc
index 129532e91f..e6c5cf2408 100644
--- a/onnxruntime/core/providers/webnn/builders/impl/unary_op_builder.cc
+++ b/onnxruntime/core/providers/webnn/builders/impl/unary_op_builder.cc
@@ -48,7 +48,7 @@ Status UnaryOpBuilder::AddToModelBuilderImpl(ModelBuilder& model_builder, const
   } else if (op_type == "Neg") {
     output = model_builder.GetBuilder().call<emscripten::val>("neg", input);
   } else if (op_type == "Not") {
-    output = model_builder.GetBuilder().call<emscripten::val>("not", input);
+    output = model_builder.GetBuilder().call<emscripten::val>("logicalNot", input);
   } else if (op_type == "Reciprocal") {
     output = model_builder.GetBuilder().call<emscripten::val>("reciprocal", input);
   } else if (op_type == "Sin") {