diff --git a/cmake/CMakeLists.txt b/cmake/CMakeLists.txt
index ed002cc0df..f7324cee09 100644
--- a/cmake/CMakeLists.txt
+++ b/cmake/CMakeLists.txt
@@ -312,7 +312,7 @@ endif()
 
 if (onnxruntime_BUILD_WEBASSEMBLY)
   # Enable LTO for release single-thread build
-  if (NOT CMAKE_BUILD_TYPE STREQUAL "Debug" AND NOT onnxruntime_ENABLE_WEBASSEMBLY_THREADS)
+  if (NOT CMAKE_BUILD_TYPE STREQUAL "Debug")
     # NOTES:
     # (1) LTO does not work for WebAssembly multi-thread. (segment fault in worker)
     # (2) "-flto=thin" does not work correctly for wasm-ld.
diff --git a/cmake/onnxruntime_unittests.cmake b/cmake/onnxruntime_unittests.cmake
index 26da6d525c..8615982519 100644
--- a/cmake/onnxruntime_unittests.cmake
+++ b/cmake/onnxruntime_unittests.cmake
@@ -669,15 +669,7 @@ if(WIN32)
 endif()
 
 if (onnxruntime_BUILD_WEBASSEMBLY)
-  if (onnxruntime_ENABLE_WEBASSEMBLY_THREADS)
-    # WebAssembly threading support in node is an experimental feature yet
-    # and that makes some intensive threadpool tests fail randomly.
-    # Will enable this test when node.js releases a stable version supporting multi-threads.
-    list(REMOVE_ITEM all_tests
-      "${TEST_SRC_DIR}/platform/threadpool_test.cc"
-      "${TEST_SRC_DIR}/providers/cpu/nn/string_normalizer_test.cc"
-    )
-  else()
+  if (NOT onnxruntime_ENABLE_WEBASSEMBLY_THREADS)
     list(REMOVE_ITEM all_tests
       "${TEST_SRC_DIR}/framework/execution_frame_test.cc"
       "${TEST_SRC_DIR}/framework/inference_session_test.cc"