diff --git a/js/web/lib/wasm/jsep/webgpu/ops/split.ts b/js/web/lib/wasm/jsep/webgpu/ops/split.ts
index 3f8131be1c..1dc3a206cf 100644
--- a/js/web/lib/wasm/jsep/webgpu/ops/split.ts
+++ b/js/web/lib/wasm/jsep/webgpu/ops/split.ts
@@ -87,7 +87,7 @@ const createSplitProgramInfo = (inputs: readonly TensorView[], attributes: Split
     previousSum += attributes.splitSizes[i];
     sizeInSplitAxis[i] = previousSum;
     const outputShape = inputShape.slice();
-    outputShape[attributes.axis] = attributes.splitSizes[i];
+    outputShape[axis] = attributes.splitSizes[i];
     outputShapes.push(outputShape);
     outputs[i] = outputVariable(`output${i}`, dataType, outputShape.length);
     outputsTensorInfo.push({ dims: outputShapes[i], dataType: inputs[0].dataType });
diff --git a/js/web/test/data/ops/split.jsonc b/js/web/test/data/ops/split.jsonc
index 46fc323cc6..f837e4d26d 100644
--- a/js/web/test/data/ops/split.jsonc
+++ b/js/web/test/data/ops/split.jsonc
@@ -64,5 +64,38 @@
         ]
       }
     ]
+  },
+  {
+    "name": "Split on Axis -1 - 2D",
+    "operator": "Split",
+    "opset": { "domain": "", "version": 12 },
+    "attributes": [
+      { "name": "axis", "data": -1, "type": "int" },
+      { "name": "split", "data": [2, 4], "type": "ints" }
+    ],
+    "cases": [
+      {
+        "name": "T[6]",
+        "inputs": [
+          {
+            "data": [1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0],
+            "dims": [2, 6],
+            "type": "float32"
+          }
+        ],
+        "outputs": [
+          {
+            "data": [1.0, 2.0, 7.0, 8.0],
+            "dims": [2, 2],
+            "type": "float32"
+          },
+          {
+            "data": [3.0, 4.0, 5.0, 6.0, 9.0, 10.0, 11.0, 12.0],
+            "dims": [2, 4],
+            "type": "float32"
+          }
+        ]
+      }
+    ]
   }
 ]