diff --git a/tools/ci_build/github/azure-pipelines/android-x86_64-crosscompile-ci-pipeline.yml b/tools/ci_build/github/azure-pipelines/android-x86_64-crosscompile-ci-pipeline.yml
index 10104641b9..2e7b403f91 100644
--- a/tools/ci_build/github/azure-pipelines/android-x86_64-crosscompile-ci-pipeline.yml
+++ b/tools/ci_build/github/azure-pipelines/android-x86_64-crosscompile-ci-pipeline.yml
@@ -4,9 +4,19 @@ jobs:
     vmImage: 'macOS-10.15'
   timeoutInMinutes: 120
   steps:
+    # Onnx has no 3.9 python package available yet, need to use python 3.8 to avoid build onnx package
+    # pythonVersion can be updated in Azure pipeline settings
+    # https://dev.azure.com/onnxruntime/onnxruntime/_build?definitionId=53
+    - task: UsePythonVersion@0
+      displayName: Use Python $(pythonVersion)
+      inputs:
+        versionSpec: $(pythonVersion)
     - script: brew install coreutils ninja
       displayName: Install coreutils and ninja
     - script: python3 tools/ci_build/build.py --android --build_dir build --android_sdk_path $ANDROID_HOME --android_ndk_path $ANDROID_HOME/ndk-bundle --android_abi=x86_64 --android_api=29 --skip_submodule_sync --parallel --cmake_generator=Ninja --build_java
       displayName: CPU EP, Build and Test on Android Emulator
     - script: python3 tools/ci_build/build.py --android --build_dir build_nnapi --android_sdk_path $ANDROID_HOME --android_ndk_path $ANDROID_HOME/ndk-bundle --android_abi=x86_64 --android_api=29 --skip_submodule_sync --parallel --use_nnapi --cmake_generator=Ninja --build_java
       displayName: NNAPI EP, Build and Test on Android Emulator
+    - script: /bin/bash tools/ci_build/github/linux/ort_minimal/nnapi_minimal_build_minimal_ort_and_run_tests.sh $(pwd)
+      # Build Minimal ORT with NNAPI and reduced Ops, run unit tests on Android Emulator
+      displayName: Build Minimal ORT with NNAPI and run tests
diff --git a/tools/ci_build/github/linux/ort_minimal/nnapi_minimal_build_minimal_ort_and_run_tests.sh b/tools/ci_build/github/linux/ort_minimal/nnapi_minimal_build_minimal_ort_and_run_tests.sh
new file mode 100644
index 0000000000..09c4dfa103
--- /dev/null
+++ b/tools/ci_build/github/linux/ort_minimal/nnapi_minimal_build_minimal_ort_and_run_tests.sh
@@ -0,0 +1,58 @@
+#!/bin/bash
+
+# This script will run a full ORT build and use the python package built to generate ort format test files,
+# and the exclude ops config file, which will be used in the build_minimal_ort_and_run_tests.sh
+
+# This script takes one command line argument, the root of the current Onnx Runtime project
+
+set -e
+set -x
+
+ORT_ROOT=$1
+MIN_BUILD_DIR=$ORT_ROOT/build_nnapi_minimal
+
+# Remove builds from previous CPU and NNAPI full Android ORT build to free up disk space
+rm -rf $ORT_ROOT/build
+rm -rf $ORT_ROOT/build_nnapi
+
+# Build with reduced ops requires onnx
+python3 -m pip install -U --user onnx
+
+# Copy all the models containing the required ops to pass the UT
+mkdir -p $TMPDIR/.test_data/models_to_include
+cp $ORT_ROOT/onnxruntime/test/testdata/ort_github_issue_4031.onnx $TMPDIR/.test_data/models_to_include
+cp $ORT_ROOT/onnxruntime/test/testdata/mnist.onnx $TMPDIR/.test_data/models_to_include
+cp $ORT_ROOT/onnxruntime/test/testdata/ort_minimal_test_models/*.onnx $TMPDIR/.test_data/models_to_include
+
+# Build minimal package for Android x86_64 Emulator
+# No test will be triggered in the build process
+# UT will be triggered separately below
+python3 $ORT_ROOT/tools/ci_build/build.py \
+    --build_dir $MIN_BUILD_DIR \
+    --config Debug \
+    --skip_submodule_sync \
+    --parallel \
+    --cmake_generator=Ninja \
+    --use_nnapi \
+    --android \
+    --android_sdk_path $ANDROID_HOME \
+    --android_ndk_path $ANDROID_HOME/ndk-bundle \
+    --android_abi=x86_64 \
+    --android_api=29 \
+    --minimal_build extended \
+    --disable_rtti \
+    --disable_ml_ops \
+    --disable_exceptions \
+    --include_ops_by_model $TMPDIR/.test_data/models_to_include/ \
+    --include_ops_by_config $ORT_ROOT/onnxruntime/test/testdata/reduced_ops_via_config.config \
+    --skip_tests
+
+# Start the Android Emulator
+/bin/bash $ORT_ROOT/tools/ci_build/github/android/start_android_emulator.sh
+
+# Push onnxruntime_test_all and testdata to emulator
+adb push $MIN_BUILD_DIR/Debug/onnxruntime_test_all /data/local/tmp/
+adb push $MIN_BUILD_DIR/Debug/testdata /data/local/tmp/
+
+# Perform the UT
+adb shell 'cd /data/local/tmp/ && ./onnxruntime_test_all'